In this research, irradiation by gamma rays was employed as an eco-friendly route for the construction of bimetallic silver-gold nanoparticles (Ag-Au NPs), while Gum Arabic polymer was used as a capping agent. Ag-Au NPs were characterized through UV–Vis., XRD, EDX, HR-TEM, FTIR, SEM/mapping and EDX analysis. Antibiofilm and antimicrobial activities were examined against some bacteria and Candida