Direct detection of biotinylated proteins (DiDBiT) is a proteomic method that can enrich and detect newly synthesized proteins (NSPs) labeled with bio-orthogonal amino acids with 20-fold improved detectability compared to conventional methods. However, DiDBiT has currently been used to compare only two conditions per experiment. Here, we present DiDBiT-TMT, a method that can be used to quantify NSPs across many conditions and replicates in the same experiment by combining isobaric tandem mass tagging (TMT) with DiDBiT. We applied DiDBiT-TMT to brain slices to determine changes in the de novo

Cotton (Gossypium spp.) is an economically important crop, but its productivity is often hindered by the soil-borne pathogen Verticillium dahliae. This study aimed to investigate the response of cotton roots to V. dahliae infection by analysing the proteome of Gossypium thurberi (resistant) and Gossypium raimondii (susceptible) at 0 h, 24 h, and 48 h post-infection. Through weighted protein coexpression network analysis, fifteen hub proteins crucial for defense against V. dahliae were identified. Expression analysis revealed the pivotal role of GthGAPC2, encoding GLYCERALDEHYDE-3-PHOSPHATE

Chickpea (Cicer arietinum) is a grain crop that is an important source of protein, vitamins, carbohydrates and minerals. It is highly sensitive to salt stress, and salt damage to cellular homeostasis and protein folding affects production. Plants have several mechanisms to prevent cellular damages under abiotic stresses, such as proteins in the endoplasmic reticulum (protein isulfide somerases (PDIs) and PDI-like proteins), which help prevent the build-up of mis-folded proteins that are damaged under abiotic stresses. In this study, we completed initial comprehensive genome-wide analysis of

Background: Systemic lupus erythematosus (SLE) is an autoimmune disease with inflammatory nature. One of the leading causes of death in SLE patients is cardiovascular (CVS) morbidity. MiRNA-24 is highly expressed in vascular endothelial cells (VECs). This dysregulated expression pattern is associated with dysfunction or even damage of VECs and leads to the occurrence of cardiovascular diseases. YKL-40 is an inflammatory glycoprotein involved in the pathogenesis of endothelial dysfunction and thereby atherosclerosis. In this work, we aimed at illustrating the possible role of miR-24 and its

Background: Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide in terms of mortality, and susceptibility is attributed to genetic, lifestyle, and environmental factors. Lymphotoxin alpha (LTA) has a crucial role in communicating the lymphocytes with stromal cells and provoking cytotoxic effects on the cancer cells. There are no reports on the contribution of the LTA (c.179 C>A; p.Thr60Asn; rs1041981) gene polymorphism to HCC susceptibility. The main aim of this study is to investigate the association of LTA (c.179 C>A; p.Thr60Asn; rs1041981) variant with the HCC

Anhydrous ammonia is a cheaper source of nitrogen (N) fertiliser than granular urea for rice production, but it is not widely used in developing countries. It can only be applied pre-crop with any in-crop applications being applied in the form of urea. This 2-year study conducted in the Nile delta region of Egypt compared pre-crop anhydrous ammonia injected to a depth of 20 cm with broadcast urea as N sources for rice, along with 4 combinations of pre-crop ammonia and in-crop urea. Each treatment supplied a total of 165 kg N/ha. The rice crop was direct seeded rather than transplanted. The

Although several studies have investigated the effect of either probiotic feed additives or postmortem meat treatment on the quality of obtained chicken meat, the impact of combined treatment with probiotic feed additives along with meat dipping in essential oil nano-emulsion on meat shelf-life is barely examined. There-fore, this study investigated the effect of combined treatment with Saccharomyces cerevisiae yeast (SCY) and rosemary oil nano-emulsion (RNE) on the quality and shelf-life of chilled broiler meat. The experimental part consisted of adding SCY as a feed additive to broiler

Aim The aim of this research was to study serum vitamin D and micro-ribonucleic acid-146a (miRNA-146a) expression in dry eye disease (DED) associated with rheumatoid arthritis (RA) and their clinical correlations to DED parameters. Setting and design This is an observational cross-sectional study that was conducted at Cairo University, Cairo, Egypt. Patients and methods The study participants were divided into three groups: group A, DED/RA (n=35); group B, non-DED/RA (n=36); and group C, non-DED/non-RA (n=35). All participants were assessed for ocular surface disease index, tear breakup time

Agricultural land expansion is a solution to address global food security challenges in the context of climate change. However, the sustainability of expansion in arid countries is difficult because of scarce surface water resources, groundwater salinity, and the health of salt-affected soil. Developing expansion and sustainability plans for agriculture requires systems thinking, considering the complex feedback interactions between saline groundwater, salt-affected soil, plant growth, freshwater mixing with saline groundwater, irrigation systems, and the application of soil amendments to

Aflatoxins are one of the most dangerous toxic residues in various foods including poultry. This study was conducted to assess the reducing effect of gamma radiation on the levels of aflatoxin B1 in poultry meat, skin, and liver. To this end, a total of 80 poultry samples including meat, skin, and liver were surveyed for the incidence of aflatoxins, where only positive samples (27 samples of muscle, skin, and liver; 9 samples each) were selected for testing the effect of treatment by gamma radiation. The levels of aflatoxins were estimated in the examined samples using High Performance Liquid